The Palmerston North Screen

Search Designs for Protein Crystallization based on Orthogonal Arrays
Kingston, R.L., Baker, H.M. and Baker, E.N.
Acta Crystallographica Section D - Biological Crystallography
50: 429-440, Part 4 JUL 1 1994

4 PEG 6000 concentrations and 4 PEG-mme 5000 concentrations (7%, 14%, 21%, 28%) screened at 8 pH values (4.9, 5.5, 6.1, 6.7, 7.3, 7.9, 8.5, 9.1).

Total number of conditions is 64 shown below as arranged for 4 4x6 trays.

	pH 4.9	pH 5.5	pH 6.1	pH 6.7	pH 7.3	pH 7.9
7% PEG 6000	7% PEG 6000 0.2M Citric acid/KOH pH 4.9	7% PEG 6000 0.2M Succinic acid/KOH pH 5.5	7% PEG 6000 0.2M Cacodylic acid/KOH pH 6.1	7% PEG 6000 0.2M PIPES/KOH pH 6.7	7% PEG 6000 0.2M MOPS/KOH pH 7.3	7% PEG 6000 0.2M EPPS/KOH ph 7.9
14% PEG 6000	14% PEG 6000 0.2M Acetic acid/KOH pH 4.9	14% PEG 6000 0.2M Malic acid/KOH pH 5.5	14% PEG 6000 0.2M MES/KOH pH 6.1	14% PEG 6000 0.2M Bis-tris propane/HCl pH 6.7	14% PEG 6000 0.2M HEPES/KOH pH 7.3	14% PEG 6000 0.2M Tris/HCL pH 7.9
21% PEG 6000	21% PEG 6000 0.2M Citric acid/KOH pH 4.9	21% PEG 6000 0.2M Succinic acid/KOH pH 5.5	21% PEG 6000 0.2M Cacodylic acid/KOH pH 6.1	21% PEG 6000 0.2M PIPES/KOH pH 6.7	21% PEG 6000 0.2M MOPS/KOH pH 7.3	21% PEG 6000 0.2M EPPS/KOH pH 7.9
28% PEG 6000	28% PEG 6000 0.2M Acetic acid/KOH pH 4.9	28% PEG 6000 0.2M Malic acid/KOH pH 5.5	28% PEG 6000 0.2M MES/KOH pH 6.1	28% PEG 6000 0.2M Bis-tris propane/HCl pH 6.7	28% PEG 6000 0.2M HEPES/KOH pH 7.3	28% PEG 6000 0.2M Tis/HCL pH 7.9

	pH 8.5	pH 9.1
7% PEG 6000	7% PEG 6000 0.2M Bis-tris propane/HCl pH 4.9	7% PEG 6000 0.2M AMPSO/KOH pH 5.5
14% PEG 6000	14% PEG 6000 0.2M TAPS/KOH pH 4.9	14% PEG 6000 0.2M Boric Acid/KOH pH 5.5
21% PEG 6000	21% PEG 6000 0.2M Bis-tris propane/HCl pH 4.9	21% PEG 6000 0.2M AMPSO/KOH pH 5.5
28% PEG 6000	28% PEG 6000 0.2M TAPSKOH pH 4.9	28% PEG 6000 0.2M Boric Acid/KOH pH 5.5

	pH 4.9	pH 5.5	pH 6.1	pH 6.7	pH 7.3	pH 7.9
7% PEG-mme 5000	7% PEG-mme 5000 0.2M Citric acid/KOH pH 4.9	7% PEG-mme 5000 0.2M Succinic acid/KOH pH 5.5	7% PEG-mme 5000 0.2M Cacodylic acid/KOH pH 6.1	7% PEG-mme 5000 0.2M PIPES/KOH pH 6.7	7% PEG-mme 5000 0.2M MOPS/KOH pH 7.3	7% PEG-mme 5000 0.2M EPPS/KOH ph 7.9
14% PEG-mme 5000	14% PEG-mme 5000 0.2M Acetic acid/KOH pH 4.9	14% PEG-mme 5000 0.2M Malic acid/KOH pH 5.5	14% PEG-mme 5000 0.2M MES/KOH pH 6.1	14% PEG-mme 5000 0.2M Bis-tris propane/HCl pH 6.7	14% PEG-mme 5000 0.2M HEPES/KOH pH 7.3	14% PEG-mme 5000 0.2M Tris/HCL pH 7.9
21% PEG-mme 5000	21% PEG-mme 5000 0.2M Citric acid/KOH pH 4.9	21% PEG-mme 5000 0.2M Succinic acid/KOH pH 5.5	21% PEG-mme 5000 0.2M Cacodylic acid/KOH pH 6.1	21% PEG-mme 5000 0.2M PIPES/KOH pH 6.7	21% PEG-mme 5000 0.2M MOPS/KOH pH 7.3	21% PEG-mme 5000 0.2M EPPS/KOH pH 7.9
28% PEG-mme 5000	28% PEG-mme 5000 0.2M Acetic acid/KOH pH 4.9	28% PEG-mme 5000 0.2M Malic acid/KOH pH 5.5	28% PEG-mme 5000 0.2M MES/KOH pH 6.1	28% PEG-mme 5000 0.2M Bis-tris propane/HCl pH 6.7	28% PEG-mme 5000 0.2M HEPES/KOH pH 7.3	28% PEG-mme 5000 0.2M Tis/HCL pH 7.9

	pH 8.5	pH 9.1
7% PEG-mme 5000	7% PEG-mme 5000 0.2M Bis-tris propane/HCl pH 4.9	7% PEG-mme 5000 0.2M AMPSO/KOH pH 5.5
14% PEG-mme 5000	14% PEG-mme 5000 0.2M TAPS/KOH pH 4.9	14% PEG-mme 5000 0.2M Boric Acid/KOH pH 5.5
21% PEG-mme 5000	21% PEG-mme 5000 0.2M Bis-tris propane/HCl pH 4.9	21% PEG-mme 5000 0.2M AMPSO/KOH pH 5.5
28% PEG-mme 5000	28% PEG-mme 5000 0.2M TAPSKOH pH 4.9	28% PEG-mme 5000 0.2M Boric Acid/KOH pH 5.5