Our major role in this project has been in determining the 3D structures using X-ray crystallography. We helped to design specific mutants to test the relative importance of the binding sites, and we assisted in the design of the bridged Pk-trisaccharide. Most of this work was carried out in the Department of Medical Microbiology and Immunology at the University of Alberta before our move to the Department of Haematology in Cambridge in 1998.
| The Read lab | University of Alberta, University of Cambridge |
|---|---|
| Amechand Boodhoo | Crystallisation |
| Penelope E. Stein | First structure of Shiga-like toxin I B-subunit |
| Hong Ling | Numerous structures of Pk-trisaccharide complexes and mutants; ligand design |
| Allan M. Sharp | Structure of F30A mutant |
| Navraj S. Pannu | Structure of starfish complex |
Our collaborators have carried out the molecular biology, protein chemistry, activity assays, carbohydrate chemistry, and cell culture toxicity assays.
| University of Alberta | University of Toronto |
|---|---|
|
Glen D. Armstrong Department of Medical Microbiology & Immunology Biological assays, Synsorb-Pk development |
James L. Brunton Departments of Medicine and Laboratory Medicine Molecular biology, protein chemistry, mutagenesis |
|
David R. Bundle Department of Chemistry Carbohydrate chemistry, Starfish design |
Dr Pavel Kitov, a post-doctoral fellow in David Bundle's group, should be singled out for his contribution in designing the five-armed Starfish inhibitor molecule.
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