Vapour Diffusion Experiment

In a vapour diffusion experiment, small volumes of precipitant and protein mixed together and the drop equilibrated against a larger reservoir of solution containing precipitant or another dehydrating agent.

Phase diagram for vapour diffusion

Hanging Drop
Trays for hanging drop experiments VDX plates, greased or ungreased, from Hampton Linbro trays from Linbro Scientific, also from Hampton or Molecular Dimensions Costar trays, from Corning/Costar, also from Hampton Q-Plate and Q-Plate II from Hampton or Molecular Dimensions Coverslips All sizes and thicknesses and siliconized or unsiliconized from Hampton. All sizes and siliconized from Molecular Dimensions DIY siliconizing rack from Molecular Dimensions. Greasing Pregreased VDX plates from Hampton Silicone sealant from Hampton or Molecular Dimensions Grease Applicator from Molecular Dimensions

Sitting Drop
Trays for sitting drop experiments As for hanging drop with microbridges from Hampton MVD/24 Crystal Growth Chambers from Charles Supper Cryschem Plates from Hampton Crystal Clears from Douglas Instruments, also available from Hampton or Molecular Dimensions Coverslips or Tape Coverslips as above Sealing tape from Hampton or Molecular Dimensions

Sandwich Drop
Trays for sandwich drops Q-Plate from Hampton and Molecular Dimensions                                                                                                                Coverslips or Tape as above

Trays for batch experiments 48 well trays from Hampton 96 well trays from Hampton or Corning/Costar For more information see Douglas Instrument's Research Papers.

Dialysis buttons are available from Hampton Trays for dialysis as for hanging drop Good Links Johan Zeelan's notes on equilibrium dialysis Hampton's Crystallization by Microdialysis

© 1999-2005 Airlie J McCoy, University of Cambridge. All rights reserved.

Last updated: 7 June, 2005