Case history: taq polymerase

• N-terminal deletion mutant

  • Stoffel fragment, like Klenow fragment of pol I

• Doubled unit cell with translational NCS

• Molecular replacement successful

• Refinement and averaging unsuccessful

  • fault of translational NCS

• Waksman group had similar crystals, but undoubled unit cell

  • they succeeded

Previous slide

Next slide

Back to first slide

View graphic version

© 1999-2005 Randy J Read, University of Cambridge. All rights reserved.

Last updated: 7 June, 2005