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Use the automated search
Don't have a quick read of the instructions and feel the need to change
the defaults. If Search for components concurrently
You should search for all components of the asymetric unit at the same time, and not in independent jobs. Search order is important The order in which you search for multiple solutions can affect your success in finding a solution. Components that account for the highest proportion of the scattering (highest completeness, highest sequence identity, and lowest B-factors) should be searched for first. If you are not sure which order is best, you can use the PERMUTATIONS ON keyword, which will search with all permutations of your input search components, but if you have a large number of different components be aware that this search will take a long time, especially if the signal from one of the components is not clear. You may need to change the packing criteria
Sometimes valid solutions are excluded because the number clashes is higher than the allowed default number.
Look in the log and summary files to see how many high Z-score
solutions You may need to change the peak selection criteria
By default, Beware the automated space group determination The space group is determined from the signal in the translation function search for the *first* model. If you have many components in the asymmetric unit, the signal from this search may be poor, and you should perform and exhaustive search in all the alternative space groups. Homology models do not have 100% identity
If you mutate the side chains of your model so that the sequence is the same as the target structure, do not enter an identity of 100% into Reduce the resolution if you can't find second and subsequent components The current algorithms have a limitation in that they do not handle the case where components of the asymmetric unit have greatly varying B-factors. This problem can be worked around by cutting the resolution to about 4 Angstroms, where the B-factor difference is less significant. Look at the FAQ list
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